DEVELOPMENT OF MODULAR PEPTIDE CARRIER FOR TARGETED GENE DELIVERY INTO HUMAN TUMOR AND STEM CELLS

DOI: https://doi.org/None

M.S. Bogacheva, А.А. Yegorova, V.S. Baranov, A.V. Kiselev D.O. Ott Research Institute of Obstetrics and Gynecology Mendeleevskaya line, 3, St. Petersburg, Russian Federation,199034

Introduction. Targeted gene delivery plays an exceptional role in the development of gene therapy. Peptide-based carriers are promising group of non-viral gene delivery vehicles due to biodegradability and easy structural and amino acid sequence modifications. The aim of the study. Study of cross-linking peptides modified with CXCR4 ligand as vehicles for gene delivery into human tumor and stem cells. Results. Here we developed non-viral gene delivery system on the basis of modular peptide carriers modified with CXCR4 ligand. Physico-chemical, DNA-protective, toxicological and transfectional properties of DNA/carrier complexes on tumor and mesenchymal stem cells were studied. Conclusion. We showed that the studied compounds are able to condence and to protect DNA against endonucleases, have low toxicity and can specifically transfect CXCR4+ cells including human mesenchymal stem cells.
Keywords: 
non-viral gene delivery, stem cells, peptide carriers, plasmid
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