CONTRACTING CAPACITY OF EMBRYONIC FIBROBLASTS WITH VARIED REGENERATIVE POTENTIAL

E.V. Sytina (1), Y.A. Chikitkina (1), T.Kh. Tenchurin (1), M.A. Paltsev (2), A.A. Panteleyev (1)
1-NRC Kurchatov Institute, Akademika Kurchatova pl. 1, Moscow, Russian Federation, 123182;
2-Institute of Biochemical Physics. N.M. Emanuel RAS, Kosygina str. 4, Moscow, Russian Federation, 119334
E-mail: a.a.pantel@gmail.com

Introduction. Contracting deep scars are almost unresponsive to conservative treatment and often require repeated surgical intervention. These scars represent a serious problem in skin therapy after the extensive damage. Just the impairment of processes of the dermis reconstitution results in the failure of healing and leads to the development of cicatricial contractions. Skin in the mammalian fetus (until the certain stage of the development) were previously shown to possess of the ability to heal without scarring, with complete restoration of the original epidermal and dermal structure, thus representing an example of true (epimorphic) regeneration. Investigation of the processes underlying this ability and its loss at the late stages of the antenatal development is of considerable interest, determined the high demand of new therapeutic approaches in the treatment of scar complications. The aim of the study. In order to identify changes in the fibroblast phenotype we assessed the contracting capacity with the use of primary mouse embryonic fibroblasts collected at various stages of the antenatal development (before and after the transition from skin regeneration to healing with scar formation). Results. Our studies demonstrated fibroblasts to show the significant shift in the contractile potential during progression from early (regenerative) to late (healing) stages of the fetal skin development.
Keywords: 
embryonic fibroblasts, scarless healing, contractility