FREE RADICAL OXIDATION OF PROTEINS AND LIPIDS IN ADIPOCYTES UNDER CONDITIONS OF OXIDATIVE STRESS

Introduction. According to the current understanding, oxidative stress plays a key role in the mechanisms of diabetes mellitus of types 1 and 2 emergence and development of its complications; formation of oxidative stress is accompanied by defective uptake of glucose by adipose tissue, reduction of insulin secretion by pancreatic B-cells, adipokines production dysregulation, progression of insulin resistance, followed by the development of metabolic syndrome. The aim of the study. Investigate the status of oxidative modification of proteins and lipids in adipocyte epididymal adipose tissue in oxidative stress. Methods. Adipocytes were isolated from the epididymal adipose tissue of rats using collagenase. The fat cells were incubated with alloxan in a concentration of 0,5–10,0 mmol/l. The content of active oxygen forms, lipid hydroperoxides, products which react with thiobarbituric acid, carbonyl derivatives of proteins, bityrosine and oxidized tryptophan in adipocytes was determined. Results. During experiments in vitro, the oxidative stress developed in adipocytes of epididymal adipose tissue when adding alloxan (0,5–10 mМ) to cell incubation medium. It was accompanied by concentration increase of oxygen active forms, products, reacting with thiobarbituric acid, hydroperoxides lipids, carbonyl derived proteins, bityrosine and oxidized tryptophan. Conclusions. In adipocyte increased generation of reactive oxygen and an increase in the intensity of oxidative modification of macromolecules leads to changes in their physical and chemical condition and infringement of functions that ultimately exacerbates diabetes and contributes to the development of complications.