ASSOCIATION OF KISSPEPTINS WITH TUMOROGENESIS IN THE FEMALE REPRODUCTIVE SYSTEM

Introduction. Kisspeptins (KP) are family of peptide mediators regulating function of gonadotrophic axis through the control of secretion of gonadotrophins at the central level. Also was shown that they are synthesized in other tissues and organs, where they have a wide range of actions. The aim of the study. In this article we investigated the expression of KP and their receptors (KISS1R) in normal ovary and in the pathology of the ovary – dermoid and endometriod cysts. Methods. The study of the fragments of endometrioid, dermoid cysts and normal ovary was performed by immunohistochemistry using the standard method with the primary monoclonal antibodies to Kiss1 (1:150, Abcam) and polyclonal antibodies to KISS1R (1:350, Abcam), with measurementt of the area and the optical density of the expression of the KP and its receptor. Results. Localization of KP and KISS1R in ovarian surface epithelium and tunica albuginea was revealed in a normal ovary and in different benign tumors of the ovary. Increased expression of KP and KISS1R in epidermal structures of dermoid cysts was detected. We demonstrated the decrease of expression of KP in the various structures of the ovary with the increasing age of the patients. Conclusion. In this study was first conducted a comparison of distribution of KP and their receptors in ovaries with cystic formations compared with healthy ovaries, this findings help to better understand the pathogenesis of these diseases.